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Paper WeP207.4

shen, yajing (Nagoya University), Nakajima, Masahiro (Nagoya University), Kojima, Seiji (Nagoya University), Homma, Michio (Nagoya University), Ode, Yasuhito (Nagoya university, Japan), Fukuda, Toshio (Nagoya University)

Characterization of Oscillating Nano Knife for Single Cell Cutting by Nanorobotic Manipulation System Inside ESEM

Scheduled for presentation during the Regular Sessions "Micro-Nano Robots and Applications to Life Science" (WeP207), Wednesday, May 11, 2011, 16:10−16:25, Room 5B

2011 IEEE International Conference on Robotics and Automation, May 9-13, 2011, Shanghai International Conference Center, Shanghai, China

This information is tentative and subject to change. Compiled on December 8, 2019

Keywords Micro/Nano Robots, Automation in Life Sciences: Biotechnology, Pharmaceutical and Health Care, Mechanism Design of Manipulators

Abstract

In this paper, a novel oscillating nano knife for single cell cutting at its native state was proposed. The nano knife with a small edge angle 5? was fabricated from a commercial AFM cantilever by focused ion beam (FIB) etching. The spring constant of the nano knife was calibrated by theoretical calculation and nanorobotic manipulation methods respectively. A piezo actuator was employed to generate the vibration of the nano knife. The oscillating amplitude response to the applied voltage was calculated based on the realtime image bservation. Single yeast cell (W303) cutting using oscillating nano knife was performed based on the nanorobotic manipulation system inside an Environment-SEM. The maximum cutting forces and the slices angle were measured from the deformation of the nano knife beam and the single cell. The result showed the oscillating nano knife was capable and effective for single cell cutting task.

 

 

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