ICRA'09 Paper Abstract

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Paper FrB9.5

Ichikawa, Akihiko (National Inst. of AIST), Tanikawa, Tamio (National Inst. of AIST), Matsukawa, Kazutsugu (NILGS), Takahashi, Seiya (NILGS), Ohba, Kohtaro (National Inst. of AIST)

Fluorescent Monitoring Using Microfluidics Chip and Development of Syringe Pump for Automation of Enucleation to Automate Cloning

Scheduled for presentation during the Regular Sessions "Micro/Nano Robotics - II" (FrB9), Friday, May 15, 2009, 11:50−12:10, Room: 501

2009 IEEE International Conference on Robotics and Automation, May 12 - 17, 2009, Kobe, Japan

This information is tentative and subject to change. Compiled on January 21, 2022

Keywords Micro/Nano Robots, Medical Robots and Systems, Manipulation Planning

Abstract

We have developed a novel technique for fluorescent monitoring of a bovine oocyte nucleus for an automatic cloning device. Animal cells that have been chemically softened by cytochalasin and stained with Hoechst dye are aspirated into a thin microchannel of a microfluidic chip and stretched thin, allowing the nucleus of the expanded oocyte to be monitored. Half the volume of the oocyte is aspirated into the thin microchannel and a high-velocity fluid flow is generated in the wide microchannel to bisect the oocyte. Then, the half-oocytes are monitored to determine which contains the nucleus. To control flow velocity with high accuracy and rapid response, we also developed a syringe pump that is small, has no backlash, and has highly-accurate volume control and a good response for automatic cutting. In this report, we describe the monitoring method and construction of the syringe pump.

 

 

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